(AB) Lactose Broth, 500 grams

Lactose Broth is prepared according to the formulation specified by the APHA for use as a fermentation broth medium in the microbiological analysis of food, milk products and water. The broth is used to detect and/or verify the presence of lactose fermenting, Gram negative, aerobic microorganisms. In the examination of milk products Lactose Broth is used only for the completed test. The determination and confirmation of coliforms in water other than drinking water is performed by using Lactose Broth. Lactose Broth provides good growth characteristics for coliforms and fermentation is observed by gas bubbles present in an inverted fermentation tube. The concentration of media prepared for the test should be adjusted to accommodate for a volume of sample greater than 10 mL. Broth medium used for the detection and/or verification of the presence of coliform organisms in water, foods, and dairy products (Alpha Biosciences # L12-105-500gm)

Use: Broth medium used for the detection and/or verification of the presence of coliform organisms in water, foods, and dairy products

Description: Lactose Broth is prepared according to the formulation specified by the APHA for use as a fermentation broth medium in the microbiological analysis of food, milk products and water. The broth is used to detect and/or verify the presence of lactose fermenting, Gram negative, aerobic microorganisms. In the examination of milk products Lactose Broth is used only for the completed test. The determination and confirmation of coliforms in water other than drinking water is performed by using Lactose Broth. Lactose Broth provides good growth characteristics for coliforms and fermentation is observed by gas bubbles present in an inverted fermentation tube. The concentration of media prepared for the test should be adjusted to accommodate for a volume of sample greater than 10 mL

Formula* per Liter:

• Pancreatic Digest of Gelatin ....... 5.0g
• Beef Extract ................................. 3.0g
• Lactose .......................................... 5.0g

.Final pH: 6.9 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 13 grams of the medium in one Liter of purified water until evenly dispersed. Distribute and autoclave at 121.0°C for 15 minutes.

Inoculum               Amount of.                     Vol of Medium                     Lactose Broth

                          Medium in tube                    and Inoculum                      Used per 1L

1 mL                10 mL or more                             11 mL                                     13.0g

10 mL                   30 mL                                      40 mL                                     17.3g

10 mL                   20 mL                                      30 mL                                     19.5g

100 mL                 50 mL                                    150 mL                                      39.0g

100 mL                 35 mL                                    135 mL                                      49.4g

100 mL                 20 mL                                    120 mL                                      78.0g

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is light yellow and clear..
3. Expected cultural response after 18-24 hours at 35.0°C.

Organism                                                                                    Result

Enterobacter aerogenes ATCC 13048                                  Growth, Gas +

Enterococcus faecalis ATCC 29212                                      Growth, Gas -

Escherichia coli ATCC 25922                                                Growth, Gas +

Proteus mirabilis ATCC 12453                                               Growth, Gas -

Pseudomas aeruginosa ATCC 27853                                    Growth, Gas -

Salmonella typhimurium ATCC 14028                                    Growth, Gas -

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.