Use: Broth medium recommended for the detection of coliforms in water, wastewater and foods.
Formula* per Liter:
Final pH: 6.8 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 35.6 grams of the medium in one Liter of purified water until evenly dispersed. Prepare double strength broth for evaluating 10 mL samples. Distribute into test tubes containing inverted
Quality Control Specifications:
Organism Result
Enterobacter aerogenes ATCC 13048 Growth, Gas +
Escherichia coli ATCC 25922 Growth, Gas +
Proteus mirabilis ATCC 12453 Growth, Gas -
Salmonella typhimurium ATCC 14028 Growth, Gas -
Staphylococcus aureus ATCC 25923 No Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.